急性放射性肺损伤大鼠模型建立的探讨

发表时间：2014-04-23 浏览次数：946次

引用：

穆懿，罗世政，柏玉举等.急性放射性肺损伤大鼠模型建立的探讨[J].吉林医学,2014,35(12):2499-2500

关键词：

急性放射性肺损伤；大鼠；模型制作

作者：

穆懿，罗世政，柏玉举，马虎

作者单位：

遵义医学院附属肿瘤医院，贵州遵义 563003

出版年份：

2014

期刊页数：

2499-2500

收录者：

万方

摘要：

目的：探讨急性放射性肺损伤大鼠模型建立的研究。方法：SD大鼠40只随机分为10 Gy组、20 Gy组、30 Gy组以及对照组(不照射)，每组10只。于直线加速器下行单次全胸5 Gy、10 Gy以及15 Gy照射，2次/周。于照射后第2周、第4周行CT扫描后随机处死5只，行右肺HE染色观察其病理变化。结果：4周内无大鼠死亡，于照射2周后总量20 Gy、30 Gy组均出现不同程度的皮毛倒翻、无光泽，呼吸急促，以及饮食、体重减少等情况，其中30 Gy组最为明显。病理切片、胸部CT显示20 Gy、30 Gy照射组于第2周、第4周均有不同程度的急性炎性反应，其中以30 Gy组最为显著。结论：30 Gy照射组为建立急性放射性肺损伤大鼠模型最佳照射剂量，为今后研究防治急性放射性肺损伤作用机制的筛选提供了基础。　　Objective Model of acute lung injury in rats radioactive established research.Methods SD rats were randomly divided into 40 groups 10 Gy,20 Gy group,30 Gy group and the control group(without irradiation),n=10.At the downstream single linear accelerator full chest 5 Gy,10 Gy and 15 Gy irradiation,2 times/week.In the first two weeks after irradiation,were sacrificed after 4 weeks 5 CT scan,the line of the right lung pathological changes observed by HE staining.Results No rats died within 4 weeks,2 weeks after irradiation in total 20 Gy,30 Gy group there are different degrees of fur spilled,dull,shortness of breath,as well as diet,weight loss,etc.,the most obvious of which 30 Gy group.Biopsy,chest CT showed 20 Gy,30 Gy irradiation group in the first two weeks,the first four weeks have varying degrees of acute inflammatory reaction,in which the most significant 30 Gy group.Conclusion 30 Gy irradiation group was established model of acute lung injury in rats optimal radiation dose for future studies of acute radiation-induced lung injury prevention screening mechanism provides a basis.

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