联合检测母血MMP-9、IL-6预测胎膜早破、亚临床绒毛膜羊膜炎的临床意义

发表时间：2014-01-23 浏览次数：655次

引用：

程秋蓉,向敏,陈民敬,等.联合检测母血MMP-9、IL-6预测胎膜早破、亚临床绒毛膜羊膜炎的临床意义[J].吉林医学,2014,35(2):231-232.

关键词：

MMP-9；IL-6；胎膜早破；绒毛膜羊膜炎

作者：

程秋蓉1，向敏2，陈民敬3，毛英1，张钰华4

作者单位：

（1.吉首大学第一附属医院妇产科，湖南吉首416000；2.吉首大学第一附属医院检验科，湖南吉首41

出版年份：

2014

期刊页数：

231-232.

收录者：

知网,万方

摘要：

目的：通过检测胎膜早破孕妇母血、胎膜组织中MMP-9、IL-6的表达水平，探讨其与胎膜早破、亚临床绒毛膜羊膜炎的相关性，探索预测和诊断亚临床绒毛膜羊膜炎的可行性的生物学指标。方法：ELISA方法检测胎膜早破及对照组孕妇血清MMP-9、IL-6的水平，同时取胎膜破口边缘处胎膜，采用免疫组化法（SP法）检测MMP-9、IL-6在胎膜上的表达；且病理检查确定有无组织学绒毛膜羊膜炎。结果：①胎膜早破孕妇血清MMP-9、IL-6水平明显高于正常对照组；②胎膜早破组胎膜中MMP-9、IL-6表达明显高于正常对照组；③母体血清MMP-9浓度为82.97 pg/ml时，IL-6浓度为46.38 pg/ml时，可作为预测临床绒毛膜羊膜炎的阈值。结论：MMP-9、IL-6可作为预测胎膜早破、合并亚临床绒毛膜羊膜炎的可行性生物学指标，联合检测更有利于早期诊断和预测。 ObjectiveThrough detect the expression of MMP-9,IL-6 in maternal serum and fetal membrane in pregnant woman with premature rupture of membranes,to approach the relationship between them,premature rupture of membranes and subclinical chorioamnionitism,the feasibility of biological indicators to anticipate and diagnose subclinical chorioamnionitis.MethodsWomen with premature rupture of membranes and normal women were studied,expression of MMP-9,IL-6 in maternal serum were measured by enzyme-linkedimmunosorbent assay. At the same time,the expression of MMP-9,IL-6 in fetal membranes,which collected from the edge of fetal membranes rupture membranes,were detected by immunohistochemical method(SP). Chorioamnionitis were diagnosed by histopathologically　Results① The lever of MMP-9,IL-6 in maternal serum in pregnant woman with premature rupture were significantly higher than that in the control group. ②The lever of MMP-9,IL-6 in fetal membranes were significantly higher in pregnant woman with premature rupture of membranes than in women in controls.③Maternal serum MMP-9 lever 82.97 pg/ml ng/ml,IL-6 lever 46.38 pg/ml ng/ml were regarded as the diagnosis threshold value of the subclinical chorioamnionitis.Conclusionserum MMP-9,IL-6 lever is a useful biological indicators for early detection of premature rupture of membranes and subclinical chorioamnionitis. And Joint detection is more conducive to early diagnosis and prediction.

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